Vitamin D
MetabolismAlso known as: 25-OH vitamin D, 25-hydroxyvitamin D, calcidiol, cholecalciferol
Vitamin D is a fat-soluble vitamin that acts like a hormone in the body.
It's essential for calcium absorption, bone health, immune function, and muscle performance. The body can produce it through sunlight exposure, but it can also be obtained from food and supplements.
Reference range
Source: Ahead Health benchmark
Reference ranges may vary between labs and assays. Always interpret results with your healthcare provider.
Why this matters
Low vitamin D can lead to weakened bones (osteopenia or osteoporosis), muscle weakness, and increased risk of falls. It may also affect immune function and mood. Maintaining adequate vitamin D levels through sunlight, diet, and supplementation when needed supports bone strength, muscle function, and overall health.
How this connects to other biomarkers
- Low Vitamin D with low Calcium and elevated Alkaline Phosphatase suggests soft, weakened bones (osteomalacia) or a compensating overactive parathyroid (secondary hyperparathyroidism).
- Vitamin D is needed to absorb calcium from the gut - persistently low Albumin-corrected Calcium despite oral calcium supplementation should prompt vitamin D testing.
- In chronic kidney disease (low estimated Glomerular Filtration Rate (eGFR)), the kidneys can no longer activate vitamin D properly, so 25-OH levels often drop.
How often should I test Vitamin D?
Most adults benefit from a yearly vitamin D check, ideally in late winter when stores are lowest. After starting supplementation, retest at 3 months. Once at target, annual checks are usually enough to maintain.
At baseline / for screening: Once every 12 months, ideally in late winter when stores are lowest. More frequently if you have malabsorption, take medications that affect vitamin D metabolism, or have a high BMI (body fat sequesters vitamin D).
When monitoring an intervention or change: Retest 3 months after starting or adjusting vitamin D supplementation, since that's how long blood levels take to stabilize at a new steady state. Compare values from the same time of year for a meaningful trend, since season and sun exposure shift levels substantially.
Note: Vitamin D status shifts with season, sun exposure, and BMI. For a meaningful trend, compare values from the same time of year (late winter to late winter); mixing seasons obscures the real picture. After starting supplementation, the 3-month retest establishes your individual dose-response.
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